thermo Qubit ssDNA Assay Kit

thermo Qubit ssDNA Assay Kit

thermo Qubit ssDNA Assay Kit

Qubit熒光計工作原理
單個(ge) 樣品檢測非常簡單
便於(yu) 使用的熒光計，可快速簡單地獲得實驗結果 大尺寸的彩色觸摸屏，可輕鬆進行各種不同操作。 計算和設置都由儀(yi) 器自動完成 (圖 1)。 Qubit 4熒光計可保存高達1000個(ge) 樣品的數據，數據可由USB數據線或U盤傳(chuan) 輸。 此外，新的Qubit 4還包含一個(ge) 內(nei) 置的試劑計算器，可以輕鬆確定準備樣品和標準所需的染料和緩衝(chong) 液的量 （圖2）。

適用於(yu) Qubit熒光計的試劑盒
用於(yu) Qubit熒光計的Qubit試劑盒，配合簡單的混合讀取模式，均采用相同的操作方式。其中DNA和RNA分析試劑盒僅(jin) 需孵育2分鍾，而蛋白分析試劑盒需要15分鍾 (圖 3)。 Qubit 4熒光計自動保存您的數據，您可用USB數據線或U盤導出。 除了常規的 dsDNA，寡核苷酸，總RNA，小RNA和蛋白質的分析試劑盒，在 Ion Personal Genome Machine(PGM) 測序儀(yi) 上進行測序之前，可使用專(zhuan) 門的Ion Sphere Quality Control Kit在Qubit 4熒光計上進行Ion Sphere顆粒質量評估。

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for ssDNA quantitation?
• For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Sample Type (General): ssDNA

For Use With (Equipment): Qubit® Fluorometer

Quantitation Range: 1-200 ng

Number of Reactions: 100 reactions

Product Line: Quant-iT™

Product Size: 1 kit

Shipping Condition: Room Temperature

內(nei) 容及儲(chu) 存

Store in refrigerator (2–8°C) and protect from light.

Note: 500 µL thin-walled PCR tubes are required but not included.