詳細介紹
品牌 | 其他品牌 | 供貨周期 | 現貨 |
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Expedeon InstantBlue蛋白電泳染色劑
Expedeon InstantBlue蛋白電泳染色劑
Expedeon 公司是英國一家專(zhuan) 注於(yu) 生物偶聯技術(bioconjugation)的企業(ye) 。生物偶聯即將兩(liang) 種分子結合在一起而形成一個(ge) 雜合的偶聯物(hybrid conjugate),這些偶聯結合物(如標記抗體(ti) )在世界各大醫院和科研機構被廣泛使用。Expedeon 公司開發生產(chan) “一步法”抗體(ti) 標記試劑盒-Lightning-Link™係列產(chan) 品極大程度的簡化了結合標記流程,是目前世界上操作較簡便的抗體(ti) 標記試劑盒。
米兰俱乐部足球推荐计划擁有強大的采購係統。隻要您想得到。沒有公司采購辦不到的。
米兰俱乐部足球推荐计划擁有完善的售後平台。隻要有售後。我們(men) *時間為(wei) 您解決(jue) 。
米兰俱乐部足球推荐计划直接與(yu) 廠家合作。保證貨源正品。購買(mai) 無後顧之憂。
米兰俱乐部足球推荐计划不保證100%。但保證100%產(chan) 品都是正品。
我們(men) 長期與(yu) Thermo Bio-rad Axygen qiagen Corning BD Promega Biotium BV targetmol merckmillipore Sigma AAT-Biolite biorbyt ENZO GE Greiner Immonoway Jackson leagene nunc Omega OmnimAbs PMG RD ZOMANBIO 聚仕隆serum 普羅麥格 金鍾 Eaivelly阿拉丁,麥克林,TCI LKT Laboratories,Epigentek,Biovision,Abnova Oxoid等各*保持合作。
張經理:Q821536999
米兰俱乐部足球推荐计划銷售銷售
在進行多因子檢測時,我們(men) 往往因不能找到多種合適的標記一抗,而不得不選用標記二抗,因此很難避免可能發生的交叉反應及繁瑣的洗滌步驟。Lightening-LinK™抗體(ti) 標記試劑盒係列,提供多種熒光蛋白、酶及熒光素標記等產(chan) 品,試驗操作人員可直接應用於(yu) 標記一抗,從(cong) 而避免了因使用二抗帶來的交叉反應及非特異性結合等問題。該係列試劑盒具有以下顯著特點
特點 優(you) 勢
● 操作簡單:一步法標記
● 快速: 手工操作時間僅(jin) 30秒 ● 目前世界上操作較簡便的抗體(ti) 標記試劑盒
● 無需脫鹽、透析等步驟 ● 抗體(ti) 回收率達100%
● 可提供50種標記物 ● 可用於(yu) WB,IHC,ELISA,免疫熒光、流式細胞術以及熒光共振能量轉移檢測等
● 經濟實惠,規格多樣 ● 可標記ug-mg 級抗體(ti) 量
Lighting-Link™係列與(yu) 其他品牌的抗體(ti) 標記試劑盒比較
廠 家 Supplier I1 Supplier I2 Supplier I3 Supplier S1 Supplier S2 Supplier D Lighting-Link
手工操作時間 2 分鍾 20 分鍾 30 分鍾 30 分鍾 30 分鍾 10 分鍾 30 分鍾
是否過柱 不需要 需要 需要 需要 需要 需要 不需要
試劑盒組分 6 組分 7 組分 5 組分 9 組分 612 組分 6 組分 3 組分
操作步驟 5 步 11 步 16 步 27 步 31 步 8 步 3 步
抗體(ti) 標記時間 10 分鍾 2.5 小時 2 小時 1.5 小時 4 小時 1 小時 2 小時
可標記抗體(ti) 量 1-20ug 10-20ug 20-100ug 25ug-5mg 100ug 50-200ug 100ug-5mg
連接化學健 非共價(jia) 結合* 共價(jia) 結合 共價(jia) 結合 共價(jia) 結合 共價(jia) 結合 共價(jia) 結合 共價(jia) 結合
抗體(ti) 亞(ya) 型 僅(jin) IgG 僅(jin) IgG 所有 僅(jin) IgG 僅(jin) IgG 僅(jin) IgG 所有
*非共價(jia) 連接的抗體(ti) 標記物隻能穩定30分鍾
試劑盒組成
● 1或3(以規格而定)玻管Lightening-Link™ Mix 混合物。
● 1管LL-X(X為(wei) 標記物,如PE或HRP等)修飾試劑。
● 1管LL-X萃滅試劑。
操作步驟
1)在每10ul抗體(ti) 樣品中加入1ul的LL-Modifier修飾試劑,輕輕混勻
2)打開Ligtening-Link混合物管蓋,吸取步驟1已混好的抗體(ti) 樣品至管內(nei) 凍幹混合物中,用吸液器輕輕吸打1-2次,使粉末*溶解
3)蓋上玻璃瓶蓋,室溫(20-25°C)避光孵育3小時,或過夜
4)孵育3小時後,按每10ul抗體(ti) 的量加入1ul LL-淬滅試劑,靜置30分鍾後即可使用
*不同的標記物,操作步驟略有不同。(操作步驟供參考,以英文原版說明書(shu) 為(wei) 準
標記結合物的保存
對於(yu) 任何新結合物,推薦保持於(yu) 4°C條件下。如需長期保存,可添加保護劑
緩衝(chong) 液及添加劑兼容性:
A. 與(yu) 無胺基緩衝(chong) 液兼容,包括MES,MOPS,HEPES(濃度10-50mM, pH範圍6.5-8.5),LL-Modifier修飾劑提供結合反應的條件
B. 常見的鹽(如氯化鈉)、螯合劑(EDTA)、糖對偶聯反應無影響
C. 疊氮鈉(0.02%-0.1%)對結合反應有輕微影響,疊氮鈉不可用於(yu) HRP標記,其為(wei) 後者的抑製劑
D. 甘油(up to 50%)對結合效率無影響
E. 若無胺基的緩衝(chong) 液濃度相對較高,pH值在6.6-8.5範圍外,則需要加入更多的LL-midifier修飾劑(/10ul抗體(ti) ),以此來調整pH值。的pH範圍為(wei) 7.3-7.6左右
F. 避免使用含親(qin) 核劑的緩衝(chong) 液,避免還原劑如DTT和2-巰基乙醇,乙醇胺等
G. 濃度達到或稍低於(yu) 20mM的Tris對結合效率有輕微的影響
Lightening-Link™ 抗體(ti) /蛋白標記試劑盒
● 酶標記試劑盒
Lightning-Link® Alkaline Phosphatase (AP)
Lightning-Link® Glucose Oxidase
Lightning-Link® Horseradish Peroxidase (HRP)
● 及鏈黴親(qin) 和素標記試劑盒
Lightning-Link® Avidin
Lightning-Link® Biotin
Lightning-Link® Streptavidin
Expedeon 701-0000 Lightning-Link® HRP Labeling Kit Ambient temperature ¥4,019.10
Expedeon 701-0002 Lightning-Link® HRP Labeling Kit Ambient temperature ¥6,895.71
Expedeon VGE-1012 Gel Wrap Gasket 1mm for 14.5cm plates Room Temperature ¥196.98
Expedeon VGE-1016 Gel Wrap Gasket 1mm for 16.5cm plates Room Temperature ¥196.98
Expedeon VGE-1017 Gel Wrap Gasket 1mm for 17cm plates Room Temperature ¥196.98
Expedeon VGE-1019 Gel Wrap Gasket 1mm for 19cm plates Room Temperature ¥253.26
Expedeon VGE-1020 Gel Wrap Gasket 1mm for 22cm plates Room Temperature ¥253.26
Expedeon VGE1020-177 Gel Wrap Gasket 1mm for DGGE Room Temperature ¥295.47
Expedeon VGE-1025 Gel Wrap Gasket 1mm for 28cm plates Room Temperature ¥295.47
Expedeon VGE-1040 Gel Wrap Gasket 1mm for 38.7cm plates Room Temperature ¥351.75
Expedeon VGE-10XX Gel Wrap Gasket 1mm per foot Room Temperature ¥126.63
Expedeon VGE-1512 Gel Wrap Gasket 1.5mm for 14.5cm plates Room Temperature ¥196.98
Expedeon VGE-1516 Gel Wrap Gasket 1.5 mm for 16.5cm plates Room Temperature ¥196.98
Expedeon VGE-1517 Gel Wrap Gasket 1.5mm for 17cm plates Room Temperature ¥196.98
Expedeon VGE-1519 Gel Wrap Gasket 1.5 mm for 19cm plates Room Temperature ¥253.26
Expedeon VGE-1520 Gel Wrap Gasket 1.5mm for 22cm plates Room Temperature ¥253.26
Expedeon VGE1520-177 Gel Wrap Gasket 1.5mm for DGGE Standard conditions - no special allowances needed ¥267.33
Expedeon VGE-1525 Gel Wrap Gasket 1.5mm for 28cm plates Room Temperature ¥295.47
Expedeon VGE-1540 Gel Wrap Gasket 1.5mm for 38.7cm plates Room Temperature ¥351.75
WMHE-600 Flexicast Midi Horizontal, 16cm(w) x 16cm(l) Kit Standard conditions - no special allowances needed 訂購詢價(jia)
Expedeon WPS-100 Wedge Plate Separators Room Temperature ¥140.70
Expedeon NXB86425 RunBlue Tris-Glycine Blot Buffer - 4L (10x Concentrate) Room Temperature ¥0.00
Expedeon NXB86500 RunBlue Tris-Glycine Blot Buffer - 500 ml (10x Concentrate) Room Temperature ¥0.00
Expedeon NXB87500 InstantBlot Transfer Buffer 500ml 10X Room Temperature ¥0.00
Expedeon NXG00812 RunBlue SDS Gel 8% 12 well - 10x10 4°C ¥0.00
Expedeon NXG00827 RunBlue SDS Gel 8% 17 well - 10x10 4°C ¥0.00
Expedeon NXG01012 RunBlue SDS Gel 10% 12 well - 10x10 4°C ¥0.00
Expedeon NXG01027 RunBlue SDS Gel 10% 17 well - 10x10 4°C ¥0.00
Expedeon NXG01212 RunBlue SDS Gel 12% 12 well - 10x10 4°C ¥0.00
Expedeon NXG01227 RunBlue SDS Gel 12% 17 well - 10x10 4°C ¥0.00
Expedeon NXG01612 RunBlue SDS Gel 16% 12 well - 10x10 4°C ¥0.00
Expedeon NXG01627 RunBlue SDS Gel 16% 17 well - 10x10 4°C ¥0.00
Expedeon NXG40812 RunBlue SDS Gel 4-8% 12 well - 10x10 4°C ¥0.00
Expedeon NXG40827 RunBlue SDS Gel 4-8% 17 well - 10x10 4°C ¥0.00
Expedeon NXG41212 RunBlue SDS Gel 4-12% 12 well - 10x10 4°C ¥0.00
Expedeon NXG41227 RunBlue SDS Gel 4-12% 17 well - 10x10 4°C ¥0.00
Expedeon NXG42002 RunBlue SDS Gel 4-20% 2 well - 10x10 4°C ¥0.00
Expedeon NXG42012 RunBlue SDS Gel 4-20% 12 well - 10x10 4°C ¥0.00
Expedeon NXG42027 RunBlue SDS Gel 4-20% 17 well - 10x10 4°C ¥0.00
Expedeon QNC-700 COOLED QUADRA MINI-SYSTEM, CE, Room Temperature ¥11,818.80
Expedeon QNX-700 QUADRA MINI-SYSTEM, CE Room Temperature ¥9,412.83
Expedeon QT-100-2 QUICKTRAP VACUUM PROTECTION SYS, 2 Liter Room Temperature ¥8,990.73
Expedeon QT-100-5 QUICKTRAP VACUUM PROTECTION SYS, 5 Liter Room Temperature ¥10,271.10
Expedeon SG-125 C.B.S. LITE VERTICAL SYSTEM Room Temperature ¥7,682.22
Expedeon SG-125-02 C.B.S. LITE VERTICAL SYSTEM,CE Room Temperature ¥8,864.10
Expedeon SG-200 C.B.S. LITE VERTICAL SYSTEM Room Temperature ¥7,949.55
Expedeon SG-200-02 C.B.S. LITE VERTICAL SYSTEM,CE Room Temperature ¥9,103.29
Expedeon SG20-0417 Rectangular tooth comb 0.4mm x 17 well Standard conditions - no special allowances needed ¥731.64
Expedeon SG20-0420 Rectangular tooth comb 0.4mm x 20 well Room Temperature ¥731.64
Expedeon SG20-0428 Rectangular tooth comb 0.4mm x 28 well Standard conditions - no special allowances needed ¥745.71
Expedeon SG20-0717 Rectangular tooth comb 0.75mm x 17 well Room Temperature ¥759.78
Expedeon SG20-0720 Rectangular tooth comb 0.75mm x 20 well Room Temperature ¥759.78
Expedeon SG20-0728 Rectangular tooth comb 0.75mm x 28 well Room Temperature ¥773.85
Expedeon SG20-1017 Rectangular tooth comb 1mm x 17 well Room Temperature ¥759.78
Expedeon SG20-1020 Rectangular tooth comb 1mm x 20 well Room Temperature ¥759.78
Expedeon SG20-1028 Rectangular tooth comb 1mm x 28 well Room Temperature ¥801.99
Expedeon SG20-1517 Rectangular tooth comb 1.5mm x 17 well Room Temperature ¥759.78
Expedeon SG20-1520 Rectangular tooth comb 1.5mm x 20 well Room Temperature ¥759.78
Expedeon SGC14-2027 Standard Comb 2mm x 27 well Standard conditions - no special allowances needed ¥745.71
Expedeon SGC20-1016 Standard Comb 1.0mm x 16 well Room Temperature ¥745.71
Expedeon SGC20-1020 Standard Comb 1mm x 20 well Standard conditions - no special allowances needed ¥745.71
Expedeon SGC20-1040 Standard Comb 1mm x 40 well Room Temperature ¥998.97
Expedeon SGC20-1520 Standard Comb 1.5mm x 20 well Standard conditions - no special allowances needed ¥745.71
Expedeon SGC20-1540 Standard Comb 1.5mm x 40 well Room Temperature ¥998.97
Expedeon SGC20-2020 Standard Comb 2mm x 20 well Standard conditions - no special allowances needed ¥745.71
Expedeon SGC20-2040 Standard Comb 2mm x 40 well Room Temperature ¥998.97
Expedeon SGC26-1026 Standard Comb 1mm x 26 well Standard conditions - no special allowances needed ¥801.99
Expedeon SGC26-1051 Standard Comb 1mm x 51 well Room Temperature ¥1,097.46
Expedeon SGC26-1526 Standard Comb 1.5mm x 26 well Standard conditions - no special allowances needed ¥801.99
Expedeon SGC26-1551 Standard Comb 1.5mm x 51 well Room Temperature ¥1,097.46
Expedeon SGC26-2026 Standard Comb 2.0mm x 26 well Standard conditions - no special allowances needed ¥801.99
Expedeon SGC26-2051 Standard Comb 2.0mm x 51 well Room Temperature ¥1,097.46
Expedeon SGE50-1020 Gel Wrap Gasket, 1mm x 22cm Room Temperature ¥450.24
Expedeon SGE50-1030 Gel Wrap Gasket, 1mm x 32cm Room Temperature ¥450.24
Expedeon SGE50-1520 Gel Wrap Gasket, 1.5mm x 22cm Room Temperature ¥450.24
Expedeon SGE50-1530 Gel Wrap Gasket, 1.5mm x 32cm Room Temperature ¥450.24
Expedeon SGP20-020 Glass Plate Set, 20cm(w) x 22cm(h) Room Temperature ¥970.83
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